Method for the optoinjection of exogenous material in a recipient biological cell enclosed by a membrane, which comprises: placing a biological cell on a planar surface of a substrate, transmitting a sub-ns pulsed laser beam through a variable convergence/divergence collimator, the collimator comprising a lens with focal length tunable by means of a variable amplitude control electrical signal; focusing the laser beam in a focal spot positioned along an axial direction substantially perpendic...
Method for the analysis of spatial and temporal information of samples by means of optical microscopy, comprising the following steps: a) choosing a field of view in a sample; b) scanning the sample in the axial direction by applying an electrical control signal having a first frequency to an electrically tunable liquid lens placed in the detection path, performing a sequential acquisition, at a first acquisition frequency which is higher than the first frequency of the electrical signal, of ...
The present invention relates to a method for providing an embedded mammalian cell, comprising the steps of providing an alginate sulfate in aqueous solution; reacting the alginate sulfate to form a hydrogel in a gelation step, providing a precursor cell, and embedding the precursor cell in the sulfated alginate hydrogel in an embedding step, thus yielding an sulfated alginate hydrogel embedded cell. The invention further relates to sulfated alginate hydrogels, and cellular grafts comprising ...