Determinazione della Triiodiotironina con tecniche immunochimiche non radioisotopiche Determination of Triiodiothyronine by non radioisotopic techniques

Determination of triiodothyronine by non radioisotopic techniques. The object of this work is the comparison of three analitical methods for the determination of triiodothyronine (3,5,3-L-triiodothyronine, T3). Which constitutes a valid support for the estimation of thyroid functionality. The following methods were evaluated: — Radial Partition ImmunoAssay (STRATUS), based on a competitive immunological reaction with a sequenzial saturation methodology, and fluorescence signal. — Microparticle capture Enzyme ImmunoAssay (IMx), a fluorometric method based on a competitive immunological reaction and on thè microparticles capacity to bind T3. Enhanced luminescence (Amerlite), based on a competitive immunological reaction revealed by thè enhanced chemiluminescence. The results obtained by these three methods have shown a fair correlation.